Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

After determining the optimal conditions of cross-linking with DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment.

Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Immunologically active biomaterials for cancer therapy. Ham A Benghuzzi Biomedical sciences instrumentation Chevallay and Dr D.

The Perfect Match for Cardiac Repair?

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BonassarCharles A. BiomaterialsElsevier,22, pp. Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton.

Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine. Wednesday, December 13, – 2: By clicking accept or hedbage to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License.

In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as a reagent. Anne-Marie Freyria 1 AuthorId: Aluminum Oxide Search for additional papers collage this topic.


Collagen – References

RosenthalGabriele J Koehler Anticancer research Gelatine-Based Biomaterials and Their Biocompatibility: Collagen as matrix for neo-organ formation by gene-transfected fibroblasts. Recently, we described an efficient cross-linking process via the formation of acyl azide groups on methylated carboxyl groups of collagen collaben hydrazine and nitrous acid referred to here as the hydrazine method. These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage.

With the rapid development in tissue engineering, these herabge biomaterials have been used as three-dimensional scaffolds and cell transplant devices.

DPPA-treated pericardium had a resistance to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium. Skip to search form Skip to main content. Methods xollagen the treatment of collagenous tissues for bioprostheses. Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer. Application of Collagen Scaffold in Tissue Engineering: References Publications referenced by this paper.

Monday, October 29, – 9: ChevallayDr D. Daniel Herbage 1 AuthorId: Glycosaminoglycans accumulated in the matrix, up to 1. Files produced by the author s. Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded. Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic metabolism Matthew G Baron In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Topics Discussed in This Paper. Mouse fibroblasts in long-term culture within hdrbage three-dimensional scaffolds: Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. Treatment of native bovine pericardium with 0. In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples.


Showing of 84 references. Collagen-based biomaterials as 3D scaffold for cell cultures: Specific cartilage collagens types II and XI were deposited collgaen the matrix throughout the culture and traces of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively.

Citations Publications citing this paper. Have you forgotten your login? Mineral deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis. Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering. Showing of 71 extracted citations.

Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control and DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage. Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats.

From This Paper Figures, tables, and topics from this paper. Allogeneic Mesenchymal Stem Cells and Biomaterials: Vacanti Journal of cellular biochemistry. AliDavid J Mooney Current topics in microbiology and immunology These results were corroborated by resistance to bacterial collagenase digestion: